解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Amyloid fibrils are considered to play causal roles in the pathogenesis of amyloid-related degenerative diseases such as Alzheimer's disease, type II diabetes mellitus, the transmissible spongiform encephalopathies, and prion disease. The mechanism of fibril formation is still hotly debated and remains an important op...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21405
更新日期:2010-06-01 00:00:00
abstract::Many studies have examined consensus sequences required for protein-glycosaminoglycan interactions. Through the synthesis of helical heparin binding peptides, this study probes the relationship between spatial arrangement of positive charge and heparin binding affinity. Peptides with a linear distribution of positive ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21339
更新日期:2010-03-01 00:00:00
abstract::A new triclinic crystal structure form of porcine pancreatic procarboxypeptidase B (PCPB) was obtained at higher resolution than the previously known tetragonal crystal structure. This new crystal polymorph has allowed for a corrected, accurate assignment of residues along the polypeptide chain based on the currently ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21320
更新日期:2010-02-01 00:00:00
abstract::Recently, we isolated and purified a neutral polysaccharide (PGN) from edible fungus Pleurotus geestanus. Its structure was characterized by a range of physical-chemical methods, including high performance anion exchange chromatography, uronic acid, and protein analyses, size exclusion chromatography with ultraviolet,...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21303
更新日期:2010-02-01 00:00:00
abstract::The structure of a bacterial cell wall may alter during bacterial reproduction. Moreover, these cell wall variations, on a nanoscale resolution, have not yet fully been elucidated. In this work, Raman spectroscopy and atomic force microscopy (AFM) technique are applied to evaluate the culture time-dependent cell wall ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21315
更新日期:2010-02-01 00:00:00
abstract::To examine stabilizing effects of the base pair interaction on a protein scaffold, various peptides with L-α-amino acids bearing a nucleobase in the side chain (nucleobase amino acids; NBAs) were designed based on a G-peptide β-hairpin structure, and their conformational properties were investigated by circular dichro...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21464
更新日期:2010-01-01 00:00:00
abstract::The advent of kinetically controlled Native Chemical Ligation has permitted more efficient sequential Native Chemical Ligation reactions to take place in one pot where one or more of the peptide fragments contains an N-terminal cysteine residue and a C-terminal thioester. The reactivity of the thioester component can ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21473
更新日期:2010-01-01 00:00:00
abstract::Peptides represent a rich natural source of potential medicines with one notable pharmaceutical limitation being their relatively short duration of action. A particularly good example of this phenomenon is glucagon-like peptide 1 (GLP), a hormone of appreciable interest for the treatment of type II diabetes. In the na...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21418
更新日期:2010-01-01 00:00:00
abstract::The use of peptide carriers, termed "cell-penetrating peptides (CPPs)" has attracted much attention due to their potential for cellular delivery of hydrophilic molecules with pharmacological interest, overcoming the membrane barrier. These peptides are able to deliver attached cargos in a nontoxic manner, with the upt...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21367
更新日期:2010-01-01 00:00:00
abstract::Peptides, peptidomimetics, and peptide derivatives that self-assemble into fibrillar gels have received increasing interest as synthetic extracellular matrices for applications in 3D cell culture and regenerative medicine. Recently, several of these fibrillizing molecules have been functionalized with bioactive compon...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.21326
更新日期:2010-01-01 00:00:00
abstract::Useful methods of protein labeling via functional peptide tags have been developed in the field of proteome and chemical biology. New tag-probe pairs based on leucine zipper peptides for labeling target proteins are described. These consist of an α-helical probe peptide with an environmental-sensitive fluorescent dye ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21444
更新日期:2010-01-01 00:00:00
abstract::Cyclotides are disulfide-rich miniproteins with a circular backbone and a knotted arrangement ofdisulfide bonds. Because these plant-derived peptides are resistant to degradation and exhibit a diverse range of bioactivity they have become important agronomic and industrial objectives. They belong to a group of compoun...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.21466
更新日期:2010-01-01 00:00:00
abstract::We review the neighbor exclusion model and some of its applications to analyze the binding data of DNA-ligand complexes. We revisit the closed form of the model developed by McGhee and von Hippel in 1974, showing that this classic model can be used to help studying the behavior of DNA contour and persistence lengths w...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.21296
更新日期:2010-01-01 00:00:00
abstract::Hepcidin is a four disulfide 25-residue peptide hormone which has a central role in the regulation of iron homeostasis. To support studies on hepcidin we have sought to establish reliable and robust synthetic methods for the preparation of correctly folded materials. While correctly-folded hepcidin has good aqueous so...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.21383
更新日期:2010-01-01 00:00:00
abstract::The shed epidermis (molt) of snakes comprises four distinct layers. The upper two layers, here considered as beta-layer, contain essentially beta-keratin. The following layer, known as mesos-layer, is similar to the human stratum corneum, and is formed by thin cells surrounded by intercellular lipids. The latter layer...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21184
更新日期:2009-12-01 00:00:00
abstract::Ribonuclease A is the archetype of a functionally diverse superfamily of vertebrate-specific ribonucleases. Inhibitors of its action have potential use in the elucidation of the in vivo roles of these enzymes and in the treatment of pathologies associated therewith. Derivatives of adenosine 5'-pyrophosphate are the mo...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21158
更新日期:2009-12-01 00:00:00
abstract::The two hemoglobin structures determined by Max Perutz, the liganded R-state, which has high oxygen affinity, and the unliganded T-state with low oxygen affinity, were landmarks in molecular and structural biology (Perutz and Lehman, Nature 1968, 219, 902-909; Bolton and Perutz, Nature 1970, 28, 551-552; Perutz et al....
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21197
更新日期:2009-12-01 00:00:00
abstract::Our previous studies of the potential utility of the C(alpha)D(alpha) stretch frequency, nu(CD), as a tool for determining conformation in peptide systems (Mirkin and Krimm, J Phys Chem A 2004, 108, 10923-10924; 2007, 111, 5300-5303) dealt with the spectroscopic characteristics of isolated alanine peptides with alpha(...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21250
更新日期:2009-09-01 00:00:00
abstract::Late expression factor 4 (LEF4) is one of the four subunits of Autographa californica nuclear polyhedrosis virus (AcNPV) RNA polymerase. LEF4 was overexpressed in Escherichia coli and recombinant protein was subjected to structural characterization. Chemical induced unfolding of LEF4 was investigated using intrinsic f...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21180
更新日期:2009-07-01 00:00:00
abstract::While end capping in alpha-helices is well understood, the concept of capping a beta-hairpin is a relatively recent development; to date, favorable Coulombic interactions are the only example of sidechains at the termini influencing the overall stability of a beta-hairpin. While cross-strand hydrophobic residues gener...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21177
更新日期:2009-07-01 00:00:00
abstract::In a seminal paper, Pakula and Sauer (Nature, 1990, 344, 363-364) demonstrated that the increase in side-chain hydrophobicity has a reverse relationship with protein stability. We have addressed this problem with several examples of mutants that span at different locations in protein structure based on secondary struc...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21187
更新日期:2009-07-01 00:00:00
abstract::The natural amino acids are primarily helix breakers at the low assignment temperatures characteristic of many studies, but recent genomic analyses of thermophilic proteins suggest that at high temperatures, some breakers may become strong helix formers. Moreover, the breaker/former inventory has not been previously c...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21129
更新日期:2009-05-01 00:00:00
abstract::The existence of an "RNA world" as an early step in the history of life increases the interest for the characterization of these biomolecules. The hairpin ribozyme studied here is a self-cleaving/ligating motif found in the minus strand of the satellite RNA associated with Tobacco ringspot virus. Surface-enhanced Rama...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21143
更新日期:2009-05-01 00:00:00
abstract::Vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide-27 (PACAP27) are members of the secretin-glucagon family containing 28 and 27 residues, respectively. NMR spectroscopy studies suggest that the N-terminus exhibit consecutive beta-turns whereas the central and C-terminal parts o...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21147
更新日期:2009-05-01 00:00:00
abstract::Optical tweezers are ideally suited to perform force microscopy experiments that isolate a single biomolecule, which then provides multiple binding sites for ligands. The captured complex may be subjected to a spectrum of forces, inhibiting or facilitating ligand activity. In the following experiments, we utilize opti...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.21123
更新日期:2009-04-01 00:00:00
abstract::Despite detailed knowledge of the overall structural changes and stoichiometries of surfactant binding, little is known about which protein regions constitute the preferred sites of attack for initial unfolding. Here we have exposed three proteins to limited proteolysis at anionic (SDS) and cationic (DTAC) surfactant ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21125
更新日期:2009-03-01 00:00:00
abstract::Single-walled carbon nanotubes (SWNTs) have unique properties and are projected to have a major impact in nanoscale electronics, materials science, and nanomedicine. Yet, these potential applications are hindered by the need for sample purification to separate SWNTs from each other and from metallic catalyst and amorp...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21159
更新日期:2009-01-01 00:00:00
abstract::Protein flexibility and conformational diversity is well known to be a key characteristic of the function of many proteins. Human blood coagulation proteins have multiple substrates, and various protein-protein interactions are required for the smooth functioning of the coagulation cascade to maintain blood hemostasis...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21062
更新日期:2008-12-01 00:00:00
abstract::Natural cellulose molecules have a strong tendency of being aggregated into larger structures. Thus, the imaging of isolated individual cellulose molecules is hampered for a long time. In this work, we manage to observe, for the first time, the isolated individual natural cellulose chains on a sample surface by means ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21070
更新日期:2008-12-01 00:00:00
abstract::The differences in the absorbance in the mid infrared region (Mid IR) between normal and abnormal tissues have been shown to be a possible criterion for the detection of different forms of cancer. The present work aimed to study the effects of cell growth and DNA synthesis on the RNA/DNA ratio, which is a promising pa...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21048
更新日期:2008-11-01 00:00:00
abstract::The thermodynamics of the stacking to unstacking transitions of 24 single-stranded DNA sequences (ssDNA), 10-12 bases in length, in sodium phosphate buffer were determined from 10 to 95 degrees C, using differential scanning calorimetry (DSC). An additional 22 ssDNA sequences did not exhibit an S<=>U transition in thi...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21044
更新日期:2008-11-01 00:00:00
abstract::Intermolecular NOE experiments have been used to explore interactions of water and ethanol molecules in 35% ethanol/65% water (v/v) with the peptide Trp-cage at temperatures from 5 to 25 degrees C. Magnetic dipole-dipole cross-relaxation terms sigma(HH) (NOE) and sigma(HH) (ROE) for interaction of solvent components w...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21028
更新日期:2008-10-01 00:00:00
abstract::Circular dichroism using synchrotron radiation (SRCD) can extend the spectral range down to approximately 130 nm for dry proteins, potentially providing new structural information. Using a selection of dried model proteins, including alpha-helical, beta-sheet, and mixed-structure proteins, we observe a low-wavelength ...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21011
更新日期:2008-09-01 00:00:00
abstract::From Auricularia auricula-judae, a water soluble beta-D-glucan, named as AAG, was isolated by extraction with 70% ethanol/water solution. Its chemical structure was analyzed by gas chromatography (GC), gas chromatography-mass spectrometry (GC-MS), matrix-assisted laser desorption /ionization (MALDI)-time of flight (TO...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20971
更新日期:2008-07-01 00:00:00
abstract::Structural data produced by a 2-ns molecular dynamics (MD) simulation on Geobacillus alanine racemase (AlaR; PDB: 1SFT) was used to study hydration around the two AlaR active sites. AlaR is a crucial enzyme for bacterial cell wall biosynthesis. It has been shown previously that the potency of an inhibitor can be incre...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20893
更新日期:2008-03-01 00:00:00
abstract::Glycopeptide analogues of CSF114(Glc), modified at N-terminus with new ferrocenyl carboxylic acid and a new ferrocenyl-thiphosphino amino acid, were used to implement a new electrochemical biosensor for autoantibody detection in multiple sclerosis. The ferrocenyl moiety of these "electrochemical probes" did not affect...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20955
更新日期:2008-01-01 00:00:00
abstract::Nanoparticle based delivery systems can offer opportunities for targeting, controlled release, and enhanced stability of their drug, protein, or gene therapy payload. This study investigated the use of chitosan in combination with the ionic additives sulfobutyl-ether-7-beta-cyclodextrin (SB-CD) or SB-CD/dextran sulfat...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.21055
更新日期:2008-01-01 00:00:00
abstract::Study of model beta-hairpin peptides allows for better understanding of the factors involved in the formation of beta-sheet secondary structure in proteins. It is known that turn sequence, sidechain-sidechain interactions, interstrand hydrogen bonding, and beta-sheet propensity of residues are all important for beta-h...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20840
更新日期:2008-01-01 00:00:00
abstract::Studies of catalytically active DNA sequences have expanded considerably since the first artificial deoxyribozyme was identified in 1994. Nevertheless, the field is still quite young, and advances in both fundamental understanding and practical applications of deoxyribozymes are still developing. Deoxyribozymes that e...
journal_title:Biopolymers
pub_type: 杂志文章,评审
doi:10.1002/bip.20813
更新日期:2007-12-05 00:00:00
abstract::The (GA)(n) microsatellite has been known from previous studies to adopt unusual, ordered, cooperatively melting secondary structures in neutral aqueous solutions containing physiological concentrations of salts, at acid pH values or in aqueous ethanol solutions. To find more about the primary structure specificity of...
journal_title:Biopolymers
pub_type: 杂志文章
doi:10.1002/bip.20822
更新日期:2007-11-01 00:00:00